Journal: RSC Advances

Article Title: Enhancing Veliparib PARP1 inhibitor stability against UVC degradation via DPPG liposome encapsulation

doi: 10.1039/d5ra02652k

Figure Lengend Snippet: Evaluation of the effect of UVC irradiation on Veliparib and DPPG encapsulating Veliparib. (a) UV-vis spectra of 50 µM Veliparib at different irradiation periods; (b) UV-vis spectra of 0.5 mM DPPG + 50 µM Veliparib at different irradiation times; (c) PCA plot of UV-vis spectra of 50 µM Veliparib and of 0.5 mM DPPG + 50 µM Veliparib solutions at different irradiation times. PC1 and PC2 present 94.816 and 3.264% variance, respectively; (d) analysis of maximum absorbance at 270 nm and 295 nm for Veliparib and DPPG + Veliparib samples at different irradiation times; (e) plot of PC2 values versus irradiation time of Veliparib and DPPG + Veliparib samples; (f) analysis of normalized areas of Gaussian 2 (∼275 nm) from irradiated Veliparib and DPPG + Veliparib spectra; (g) analysis of the normalized regions of Gaussian 5 (∼330 nm) from irradiated Veliparib and DPPG + Veliparib spectra. In the graphs, the lines between points are only guidelines.

Article Snippet: Samples were placed in quartz cuvettes and irradiated with a 254 nm UVC germicide lamp (Philips TUV PL-S 5 W/2 P 1CT) at a radiance of 28.9 W m −2 .

Techniques: Irradiation

Journal: RSC Advances

Article Title: Enhancing Veliparib PARP1 inhibitor stability against UVC degradation via DPPG liposome encapsulation

doi: 10.1039/d5ra02652k

Figure Lengend Snippet: Evaluation of the effect of UVC irradiation on Veliparib's degradation process. (a) UV-vis spectra of 500 µM Veliparib solution at different irradiation periods; (b) comparative analysis of the normalized areas of UV-visible spectra Gaussians 5 (330 nm) of irradiated 50 µM and 500 µM Veliparib samples. In this graph, the lines between points are only guidelines; (c) SDS-PAGE gel analysis of the effect of irradiated Veliparib samples (500 µM) on the auto-modification activity of PARP1. M – NZYColour Protein Marker II (NZYTech); CP – positive control without inhibitor; CPi/T0 – positive control with non-irradiated inhibitor; X’ – Veliparib's irradiation (min). Colored arrows are used to identify the irradiation time points that showcase major alterations in the inhibitory capability of Veliparib and when the normalized area of Gaussian 5 attained a maximum value. These samples were subsequently analyzed by infrared spectroscopy.

Article Snippet: Samples were placed in quartz cuvettes and irradiated with a 254 nm UVC germicide lamp (Philips TUV PL-S 5 W/2 P 1CT) at a radiance of 28.9 W m −2 .

Techniques: Irradiation, SDS Page, Modification, Activity Assay, Marker, Positive Control, Spectroscopy

Journal: RSC Advances

Article Title: Enhancing Veliparib PARP1 inhibitor stability against UVC degradation via DPPG liposome encapsulation

doi: 10.1039/d5ra02652k

Figure Lengend Snippet: Infrared spectroscopy analysis of UVC irradiated Veliparib samples. (a) Complete FTIR spectra of 500 µM Veliparib at various irradiation periods; (b) FTIR spectra comprising 900 to 1350 cm −1 region; (c) FTIR spectra comprising 1275 to 1550 cm −1 region; (d) FTIR spectra comprising 1500 to 1825 cm −1 region. The code of colors displayed in (a) is the same in all four graphs.

Article Snippet: Samples were placed in quartz cuvettes and irradiated with a 254 nm UVC germicide lamp (Philips TUV PL-S 5 W/2 P 1CT) at a radiance of 28.9 W m −2 .

Techniques: Spectroscopy, Irradiation